The CPCCG Screening Core can screen 96, 384 or 1536 well formats using either
biochemical or cell-based assays, and can process over 300,000 wells per day.
Total throughput capacity will climb to over 2 million compounds per day
following the opening of Burnham’s east coast campus in Lake Nona, Florida.
We collaborate with investigators to develop robust assays suitable for high
throughput screening (HTS) formats.
CPCCG has the ability to develop and run almost any biochemical or cell-based
assay types.
We have significant expertise in studying cell death using a variety of
biochemical and cell-based assays, with particular emphasis on kinases,
phosphatases, proteases and protein-protein interactions. CPCCG is also
experienced with phenotypic assays for primary and stem cells.
Assay formats include:
- absorbance
- ELISAs
- fluorescence intensity
- fluorescence polarization
- FRET
- luminescence
- time resolved fluorescence
- TR-FRET
- BRET
- AlphaScreen
| Assay Formats |
Screening Capabilities |
Biologicial Expertise |
|
Absorbance |
Biochemical Assay Screening |
Cell
Death (Apoptosis, Necrosis etc.) |
|
Luminescence |
Cell-Based Assay Screening |
Cell
Differentiation (phenotypic assays for stem cells using fluorescent
reporters) |
|
Fluorescence Intensity |
High-Content Cell-Based Assay Screening |
Cell
Proliferation |
|
Fluorescence Polarization |
High
Throughput Microscopy/Cell Imaging |
Cell
Motility and Invasion |
| Time
Resolved Fluorescence |
Cell
Image Analysis |
Cell
Morphology |
| FRET |
Image
Algorithm Development |
Cytotoxicity Assays |
| TR-FRET |
Bacterial, Baculovirus and Mammalian Recombinant Protein Expression Systems |
Enzyme
Assays |
|
Heterogeneous Assay Formats (ex: ELISA) |
Lentivirus Technology |
Kinase
Assays |
| NMR |
Cell
Reporter Assays |
Phosphatase Assays |
|
Transmitted Light |
Cell
Line Generation |
Protease Assays |
| |
NMR-Based Screening |
ER
Stress/Chemical Chaperone Assays |
| |
Virtual
Library Design/Screening |
Protein
Translocation Assays |
| |
In
Silico Profiling |
Protein-protein Interaction Assays |
| |
|
Cardiac
myocyte Assays |
| |
|
Pancreatic beta-cell Assays |
| |
|
Neuroregeneration Assays |
| |
|
Promoter/Reporter Assays |
| |
|
Actins,
Microtubulus and Microfilament Structures |
| |
|
Cancer |
| |
|
Diabetes |
| |
|
Inflammation and Infectious Diseases |
| |
|
Nucleic
Acid-based Targets |
| |
|
Functional Genomics/cDNA/siRNA Profiling |
| |
|
PK/ADME/Toxicity Profiling |
Guidelines for assay development can be found at the
NIH National Chemical Genomics Center. Please review their Assay Guidance
Manual for detailed information.